Human Nicotine, NICOTINE ELISA Kit

Overview
The Human Nicotine ELISA Kit is a highly sensitive and specific sandwich immunoassay designed for the quantitative determination of nicotine in human serum, plasma, cell culture supernatants, ascites, tissue homogenates, and other biological fluids. With industry-leading sensitivity (0.23 ng/ml) and a broad dynamic range (0.5–100 ng/ml), this kit is the preferred choice for researchers studying smoking behavior, nicotine addiction, tobacco exposure biomarkers, and pharmacological interventions.

Key Features

• Detection Range: 0.5 – 100 ng/ml

• Sensitivity: 0.23 ng/ml – detect even trace levels of nicotine

• Precision: Intra-assay CV <10%, inter-assay consistency assured

• Sample Types: Serum, plasma, cell culture supernatant, tissue homogenate, ascites, urine, cerebrospinal fluid

• Format: 96T or 48T pre-coated plates

• Assay Time: ~1.5 hours

• Principle: Sandwich ELISA

Kit Components

• Pre-coated 96-well or 48-well microplate

• Nicotine standard (128 ng/ml)

• Biotinylated detection antibody

• Streptavidin-HRP conjugate

• Substrate solutions A & B

• Stop solution

• Wash buffer concentrate (25X)

• Detailed protocol & plate sealers

Applications
Ideal for:

• Smoking cessation and nicotine replacement therapy research

• Tobacco exposure and public health studies

• Addiction neuroscience and behavioral pharmacology

• Clinical toxicology and forensic testing

• Pharmacokinetic and drug metabolism studies

Storage & Stability

• Unopened: Store at –20°C for 1 year or 2–8°C for 6 months

• Opened reagents: Use within 1 month

• Pre-coated strips: Store at 2–8°C when not in use

Why Choose This Kit?

• Highest sensitivity in its class (0.23 ng/ml) – capture low-level nicotine exposure

• Validated for multiple sample types – serum, plasma, urine, tissue, and culture media

• Excellent reproducibility – CV ≤10% ensures reliable data across runs

• Easy-to-follow protocol – complete in under 2 hours

• Full technical support – troubleshooting guide and expert assistance included

Market Leader Advantage
Trusted by leading addiction research centers and toxicology laboratories, this kit delivers consistent, publication-ready results. Whether you are assessing smoking status, monitoring nicotine metabolism, or evaluating cessation therapies, the MDBE0111Hu provides the accuracy and sensitivity you need.

Note: For research use only. Not for diagnostic use.