MedikBio Micro Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco) Assay Kit

Overview
Unlock the secrets of plant photosynthesis with the MedikBio Micro Rubisco Assay Kit—a highly sensitive and reliable colorimetric tool designed for the quantitative measurement of Rubisco (EC 4.1.1.39) activity in plant tissues. Rubisco is the most abundant enzyme on Earth and the primary catalyst for carbon fixation in the Calvin cycle. This kit provides a simple, reproducible method to assess total Rubisco activity or separately measure cytoplasmic and chloroplastic fractions, making it indispensable for photosynthesis research, plant breeding, crop improvement, and climate change studies.

Kit Components

• Extraction Buffer I & II

• Reagent I, II, III, IV

• Working reagents (prepared fresh)

• Complete protocol with calculation formulas

Applications
Ideal for:

• Photosynthetic efficiency and carbon fixation studies

• Plant stress physiology (drought, heat, salinity)

• Crop yield improvement and breeding programs

• Chloroplast vs. cytoplasmic enzyme activity comparison

• Climate change and CO₂ assimilation research

Assay Principle
The kit measures Rubisco activity by tracking the oxidation of NADH to NAD+ at 340 nm. One molecule of ribulose-1,5-diphosphate (RuBP) combines with CO₂ to form two molecules of 3-phosphoglyceric acid (PGA), which is converted to glyceraldehyde-3-phosphate with concomitant NADH oxidation. The decrease in absorbance at 340 nm is directly proportional to Rubisco activity.

Sample Preparation

• Total Rubisco: Extract with Buffer I, homogenize, centrifuge, collect supernatant

• Cytoplasmic/Chloroplastic Separation: Differential centrifugation with Buffer I & II

Storage & Stability

• Unopened: Store at –20°C for 6 months (protect from light)

• Opened reagents: Use within recommended timeframe

• Working reagents: Prepare fresh; aliquot and store at –20°C to avoid freeze-thaw cycles

Why Choose This Kit?

• High Sensitivity & Specificity – Optimized for plant tissue matrices

• Microplate Format – High-throughput compatibility (48T/96T)

• Simple Protocol – Complete in under 2 hours

• Dual Application – Measure total, cytoplasmic, or chloroplastic Rubisco

• Comprehensive Support – Includes detailed calculation formulas and troubleshooting

Market Leader Advantage
Trusted by leading plant science laboratories and agricultural research institutes, the MDB1480 kit delivers consistent, publication-ready results. Whether you are screening for photosynthetic mutants, evaluating stress tolerance, or optimizing crop productivity, this kit provides the accuracy and reliability you need.

Note: For research use only. Not for diagnostic use.

Recommended Companion Products

• MedikBio® Micro Coenzyme I NAD(H) Assay Kit (MDB1020)

• MedikBio® Micro NADH Oxidase (NOX) Assay Kit (MDB1021)

• MedikBio® Micro NAD+ Kinase (NADK) Activity Assay Kit (MDB1022)

• MedikBio® Micro Coenzyme II NADP(H) Assay Kit (MDB1010)