MedikBio® Superoxide Dismutase (SOD) Assay Kit

Overview
The MedikBio® Superoxide Dismutase (SOD) Assay Kit provides a convenient, sensitive, and accurate colorimetric method for the quantitative determination of SOD enzyme activity in biological samples. SOD enzymes catalyze the dismutation of superoxide (O₂⁻) into oxygen and hydrogen peroxide, serving as a critical antioxidant defense in all cells exposed to oxidative stress. Aberrant SOD activity has been linked to amyotrophic lateral sclerosis (ALS), perinatal lethality, neural disorders, and cancer. This kit is an essential tool for researchers studying oxidative stress, antioxidant defense, and related pathologies.

Assay Principle
Superoxide (O₂⁻) is generated by xanthine oxidase (XO)-catalyzed reaction. The O₂⁻ reacts with WST-1 dye to form a colored product. SOD in the sample scavenges O₂⁻, reducing the amount available for the chromogenic reaction. The decrease in color intensity (OD₄₄₀ₙₘ) is directly proportional to SOD activity. This method is highly specific and avoids interferences common in other SOD assays.

Kit Components

• Assay Buffer: 20 mL

• Diluent: 20 mL

• SOD Enzyme: 120 µL

• XO Enzyme: 400 µL

• Xanthine: 600 µL

• WST-1: 600 µL

• Detailed protocol with standard curve preparation and calculation formulas

Applications
Ideal for:

• Oxidative stress and antioxidant research

• Cancer biology and tumor microenvironment studies

• Neurodegenerative disease models (e.g., ALS, Alzheimer’s)

• Cardiovascular and metabolic disorder research

• Toxicology and drug development

• Plant and microbial stress physiology

Sample Types

• Blood (serum, plasma, erythrocyte lysate)

• Cultured cells (suspension and adherent)

• Tissue homogenates (liver, brain, kidney, etc.)

• Other biological fluids

Storage & Stability

• Shipping: Shipped on ice

• Storage: Store all reagents at –20°C

• Shelf Life: 6 months from date of receipt

• Note: For research use only. Normal laboratory precautions required.

Why Choose This Kit?

• Validated Performance: Cited in peer-reviewed publications (e.g., Journal of Functional Foods, Experimental Physiology, Life Sciences)

• Simple & Robust: No washing steps minimize errors and save time

• Flexible: Suitable for manual or automated high-throughput screening

• Comprehensive: Includes all reagents and a detailed protocol for easy setup

• Cost-Effective: 100 assays per kit, ideal for large-scale studies

Market Leader Advantage
Trusted by leading oxidative stress and free radical research laboratories worldwide, the MedikBio® SOD Assay Kit delivers consistent, publication-ready results. Whether you are screening antioxidant compounds, studying disease mechanisms, or profiling SOD activity across tissues, this kit provides the sensitivity, accuracy, and convenience you need to accelerate your research.

References

1. Magana-Cerino, J. M., et al. (2020). Journal of Functional Foods. 72: 104075.

2. Nawi, A., et al. (2020). Experimental Physiology. 105(8): 1223-1231.

3. Sahn, T. D., et al. (2020). Life Sciences. 250: 117545.

Note: For research use only. Not for diagnostic or therapeutic use.